Pyrosequencing for Microbial Identification and Characterization

Metagenomic pyrosequencing and microbial identification.

BACKGROUND The Human Microbiome Project has ushered in a new era for human metagenomics and high-throughput next-generation sequencing strategies. CONTENT This review describes evolving strategies in metagenomics, with a special emphasis on the core technology of DNA pyrosequencing. The challenges of microbial identification in the context of microbial populations are discussed. The developme...

Microarray-Based Microbial Identification and Characterization

The development of molecular-based methodologies over the past two decades has dramatically improved our ability to detect microorganisms in clinical and environmental samples—enabling detection and identification within hours in many cases. However, most of these methods are only capable of monitoring individual or small groups of organisms at a time. Due to the extreme microbial diversity in ...

Identification of Microbial Communities in Open and Closed Circuit Bioelectrochemical MBRs by High-Throughput 454 Pyrosequencing

Two bioelectrochemical membrane bioreactors (MBRs) developed by integrating microbial fuel cell and MBR technology were operated under closed-circuit and open-circuit modes, and high-throughput 454 pyrosequencing was used to investigate the effects of the power generation on the microbial community of bio-anode and bio-cathode. Microbes on the anode under open-circuit operation (AO) were enrich...

Analysis of Microbial Diversity by Amplicon Pyrosequencing

Short pyrosequencing reads suffice for accurate microbial community analysis

Pyrosequencing technology allows us to characterize microbial communities using 16S ribosomal RNA (rRNA) sequences orders of magnitude faster and more cheaply than has previously been possible. However, results from different studies using pyrosequencing and traditional sequencing are often difficult to compare, because amplicons covering different regions of the rRNA might yield different conc...